Carlos Rumbo

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Questions related from Carlos Rumbo

Pollutant extraction from contaminated soils for toxicity assays using human cell lines. How to resuspend the contaminant crude extract?

To study the toxicity of a contaminated soil with petroleum and heavy metals using human cell lines as model organism, we need to perform a pollutant extraction step before carrying out the...

09 November 2020 1,573 4 View

Why cells are dying in the centre of some random wells in a 96-well plate?

I am trying to perform a MTT assay, and I have found some unexplained problems. The protocol that we are using is the following: 1st day: Cells are seeded in 96 well plates (5000 cells per well)...

13 March 2019 3,597 17 View

Is there any alternative for Nuclease P1 to perform an 8-OHdG Quantitation?

To perform an 8-OHdG quantitation assay (with DNA extracted from eukaryotic cells as samples), an enzyme that digests DNA down to the single nucleotide level is needed. Nuclesase P1 is recommended...

25 April 2017 3,188 3 View