Carlos Martínez-Pérez

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Questions related from Carlos Martínez-Pérez

What reagent could be used to block the reducing effect of mercaptoethanol?

We've been using a new method for protein extraction (QIAGEN's Qproteome FFPE tissue) in our lab but weren't getting good results in western blots. I've realised that the extraction method...

24 February 2017 6,176 2 View

Why would a deprotonated compound be locked in the mitochondria?

I've heard from a collaborator that a compound that is likely to be deprotonated (i.e., oxidised) in the mitochondrial matrix due to its electrochemical profile would probably be locked in this...

17 August 2016 9,383 3 View

Suggestions of methods for mitochondrial protein isolation? A good loading control for mitochondrial fraction?

Hi, I'm planning to prepare cell lysates to obtain mitochondrial and cytosolic protein fractions from adherent breast cancer cells. I've been looking into abcam's fractionation kit but was...

05 February 2016 7,200 3 View

What is the relation between small Mafs and the keap1-Nrf2 activation of ARE?

What we know: It's been established that keap1 is involved in the regulation of transcription factor Nrf2, facilitating its ubiquitination and degradation in basal conditions (either by...

20 January 2016 7,790 6 View

Is Rhodamine 123 a good method to study Electron Transport Chain modulation?

Numerous sources suggest rhodamine 123 as the best probe for the study of ETC modulation using a fluorescent platereader. Is this a suitable method? I've also read that MDR transporters can target...

01 December 2014 9,999 3 View