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Hello, I identified the +1 of a given transcript using classical primer extension with radioactivity. However, the procedure is arduous, especially because most of people of my lab cannot work...
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I am trying to identify the +1 in the transcription from one promoter. To do so, I am performing 5' RACE. I extract RNA, make cDNA (both with specific primer and with random hexamers), add a poliA...
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