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Questions related from Carlos Cordon-Obras
Hello, I identified the +1 of a given transcript using classical primer extension with radioactivity. However, the procedure is arduous, especially because most of people of my lab cannot work...
05 December 2016 4,439 4 View
I am trying to identify the +1 in the transcription from one promoter. To do so, I am performing 5' RACE. I extract RNA, make cDNA (both with specific primer and with random hexamers), add a poliA...
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I performed a Chip-Seq analysis using a pol II antibody and it yielded some interesting sequences where this protein is accumulated. In order to check if these sequences could be acting as...
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