Carlos Henrique Camargo

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Questions related from Carlos Henrique Camargo

Has someone experience in using Thermo Scientific FastDigest BcuI restriction enzyme for P. aeruginosa PFGE?

Hi everyone! I have used 10U of SpeI (NEB or Promega) in restriction of P. aeruginosa plugs for PFGE, but we have now utilized the Thermo Scientific FastDigest BcuI restriction enzyme and the...

28 March 2016 2,695 2 View

How can one determine the plasmid size (in Dalton) according to kb or bp gel markers?

I would like to determine plasmid sizes in Dalton but I do not have reference strains with standard plasmid to make the inference. By using Lambda PFG marker, for instance, is it possible to...

07 March 2015 9,954 2 View