Carlo Breda

3 Questions 2 Answers 0 Followers

Questions related from Carlo Breda

Does someone have a nice detailed protocol for extracting transporter from the membrane for Western Blot?

Hi Guys, I need a detailed protocol for trying to extract an overexpressed transporter for HEK293T cells [from the plasma membrane]. So far I did use the NP-40 buffer but I guess with very bad...

06 June 2016 7,854 1 View

Can rat tail callagen II be reused?

Hi, I need to coat some plate with rat tail collagen II. I was wondering whether I can reuse the collagen after I do the first coating.... if so, how many times? Thank you in advance

05 May 2016 5,333 3 View

Does an alternative method exist for measuring riboflavin uptake without using radiolabbeling?

Hi guys, I need to study the uptake of riboflavin in some cell models. I was wondering whether anyone can suggest me a way to measure the level of uptake riboflavin without using radiolabeled...

01 January 2016 5,583 2 View