Which method can be used to do cas9 casette (promoter, Cas9, Terminator) for the Crispr-cas9 approach ?

Hello researchers I'd like to know what other method can be used to assemble 3 different DNA sequences, apart from the recombination method in saccharomyces cerevisiae, Gibson assembly, and PCR...

17 July 2023 7,424 1 View