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Using a MUT vs parent cell line, my colleagues and I have seen increased proliferation in MUT cells. To address this further, we have been synchronizing cells 48h in 0.1% serum (with 10mM sodium...
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I am wanting to look at the ratio of nuclear vs mitochondrial DNA in the human cell line MCF7 cells Using qPCR (syber green and a rotogene machine using a total cell pellet DNA). I’ve designed and...
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I'm doing a protein precipitation protocol using TCA (trichloroacetic acid) for the first time. My protocol says to dissolve 500g TCA into 350ml dH20 (and store at room temp) for a 100% (w/v)...
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