Cara L Nicholson

2 Questions 2 Answers 0 Followers

Questions related from Cara L Nicholson

How to stop sperm cells clumping during fixation?

I've been optimising a flow cytometry protocol to assess intracellular proteins in human sperm. Most published methods describe fixing with 4%PFA after washing and pelleting the cells. I am...

07 September 2020 3,008 2 View

Using ImageJ to measure area of expression of two fluorescent markers together?

Hi, I have been using ImageJ to measure the area of fetal ovaries, with Cleaved Caspase 3 and mvh/ddx4 expression for my IF. mvh is the green channel and CC3 is on the red channel. I haven't had a...

25 June 2019 3,739 3 View