How can I increase the viability of CD40L-activated B cells?

I am using the following protocol to generate B cells from PBMC:Culture PBMC with CD40L (100 ng/ml), IL-4 (2 ng/ml), Insulin (5 ug/ml), Transferin (50 ug/ml) and Cyclosporin a (2 ug/ml). Change...

17 August 2016 9,938 5 View