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Hello, we are trying to analyse the reducing sugar content in potato. System itself (except mobile phase) used to have quite serious trouble with air bubbles, yet we somehow tried to solve this...
09 September 2017 7,611 7 View
The samples we have are from leaves but dried completely at dark and in closed room. We have followed several extraction steps that included kit, kit with PVP and CTAB. However, the thing that we...
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Dear colleagues, I am analyzing reducing sugar content of several breeding lines of potato in HPLC. The mobile phase I am using is 80:20 acetonitrile:water and I sonicate it for 5 min to get rid...
03 March 2016 1,605 4 View
Hello, Due to high number of genotypes to be analyzed, I prefer to prepare 2 liters of mobile phase for daily use(acetonitrile:water 80:20) and I prepare it by weighing acetonitrile and water...
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12 December 2015 6,949 2 View
The primers we are using are also the ones validated in several studies and PCR condition is very specific (touchdown). Thanks for your time.
06 June 2015 8,512 13 View
