Camille Trautman

2 Questions 1 Answers 0 Followers

Questions related from Camille Trautman

What is this ~35 kD contaminant band in my exosome Western blots?

I have been trying to run Western blots for ALIX and CD63 using SBI's antibodies, but I get big, bright bands at ~35 kD for all of my samples. The samples were isolated using SBI's ExoQuick ULTRA...

09 September 2019 6,461 3 View

How to use less antibody in a Western blot?

I'm trying to figure out how to not need so much antibody for our Western blots. Right now, we use 10 mL of buffer to dilute our antibodies in. Any less than that, and the membrane isn't submerged...

08 August 2019 9,414 4 View