4 Questions 5 Answers 0 Followers
Questions related from Callum McKenzie
I recently purchase some A431 cells from ATCC and the recommended media is DMEM + 10% FBS (my media is also supplemented with GlutaMAX). After thawing and passaging some cells attach, however the...
10 October 2016 3,837 4 View
I would like to immortalise primary MEFs. I have conflicting approaches for how best to do this. First is to take the MEFs through oxidative stress by incubating at atmospheric oxygen levels....
10 October 2016 1,826 1 View
I am co-transfecting NIH3T3 cells with two plasmids (rasV12 mutant + gene of interest) for a transformation assay. My question is: how much reagent (FuGENE HD) should I use? I typically use a 3:1...
02 February 2016 7,624 6 View
I have been culturing these cells for around 2 weeks and have passed them twice. They never reached confluency. I am culturing them in DMEM/F12 media + 10% NCS (Sigma) + 2 mM L-Glutamine (already...
04 April 2015 6,786 3 View
