Cahya kurnia Fusianto

2 Questions 4 Answers 0 Followers

Questions related from Cahya kurnia Fusianto

What is the reason for using several time intervals to harvest a target protein (from plasmid) from E.coli?

Why, at 3, 4.5 , 6, 18 hours after IPTG induction, do we harvest the target protein from E.coli? Is there any reason for this treatment?

04 February 2013 4,389 3 View

Problem in protein expression.

I cloned my gene and sequenced it, now I have a problem in protein expression. The target protein still does not appear. Can anyone help me? I clone eucaryotic gene into e.coli using pET28a...

22 December 2012 6,822 23 View