How to overcome the issues in cloning of amiRNA(with miR155 backbone into mammalian vector(pcDNA3.1-eSIBR(intronic region containing BIC region)-GFP)?

Dear Researchers, I have custom designed mammalian vector pcDNA3.1 containing the eSIBR region that has the BIC sequence and GFP downstream to the CMV promoter. The eSIBR region has a polylinker...

12 November 2017 1,208 0 View