Byung Ho Lee

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Questions related from Byung Ho Lee

Permeability Assay - Alternative to dextran?

I am attempting to perform a permeability assay. I have tried dextran-Alexa647 10kDa. However, after long term treatment (24hrs), the cells dont seem healthy (with large vacuoles or vesciles and...

04 November 2020 1,117 0 View

I cant seem to get my head around the transformation step, any recommendations would be appreciated!?

Site mutagenesis on 11kb plasmid -optimized annealing temp (initially started with gradient pcr) -DpnI digestion carried out (checked via gel and obtained a large smear) -Transformation (Kanamycin...

18 January 2017 3,658 1 View