qRT-PCR melting curves are good for cDNA of overexpression cells but not for cDNA of parental cells. What could be the reason?

I am trying to find a good qRT-PCR primer set for a gene that has low expression in our cell lines. As you see in the attachment I have a very sharp one peak when I used cDNA from overexpression...

02 February 2020 7,805 4 View