Bryan Rogers

4 Questions 10 Answers 0 Followers

Questions related from Bryan Rogers

Kinky TNFRs - are there TNFRs with significant 'kinks' in their CRD2/3/4 domains?

Hello, I'm looking for a structure of a TNFR member with a significant kink in the middle of the CRD architecture. I am trying to compare a recently completed novel structure with other TNFRs and...

08 August 2018 5,831 0 View

How can I add more than 5 columns to an Akta Pure?

Akta Pure limits the number of columns per system to 5 due to the C-valve. What is the best way to add more columns to the system? I've heard of people using the loop valves to double the columns...

11 November 2017 2,680 3 View

True native MS analysis of a complex, what do I buffer exchange into?

I have a trimer 'thing' which is in a complex with another trimer 'thing'. These two things are different, but the mass is around 100 kD for the complex. I want to resolve the entire complex via...

10 October 2016 8,260 9 View

Akta Pure, any resources for IgG purification methods?

Hello, Much of my experience for IgG purification is in UNICORN 5.0 on an Akta Explorer and I'm looking for resources in setting up pH gradient methods for elution in UNICORN 6.4 on an Akta...

05 May 2016 9,836 7 View