Bryan Douglas Crawford

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Questions related from Bryan Douglas Crawford

How to solubilize ECM proteins in such a way as to allow IP/coIP?

All the IP protocols I've seen start with making a lysate using a buffer with mild non-ionic detergents, etc. which makes sense if you're trying to get cytoplasmic proteins into solution, and...

08 August 2018 394 5 View

I'm looking for a good protocol for imaging the ultrastructure of the ECM in fish embryos; anyone tried ruthenium red or other tricks?

We're getting reasonably good results using standard Karnofsky's fixation, OsO4, uranyl acetate & lead citrate staining, but I've heard of people using other stains - particularly ruthenium...

11 November 2016 1,393 2 View