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Questions related from Bryan John Magoling
Hello everyone! I'm currently trying to increase the expression of my fusion protein in a C. elegans animal model. After reading papers, I found two possible methods to try: (1) codon-optimization...
30 September 2020 615 2 View
Hi everyone. I have been trying to express my fusion protein to the outside membrane of the cell. My fusion protein contains eGFP. After lentivirus transduction, I observed a membrane-bound...
26 September 2020 4,926 2 View
Has anyone had previous success in amplifying Lactadherin from the addgene construct below? https://www.addgene.org/46830/ I'm looking at the sequence and it seems that the starting sequence of...
12 October 2019 1,278 3 View
Does anyone know how moisture content can affect the activation process using microwave? For example, if I put char that is not completely dry and subject it to MW treatment, will the resulting...
12 April 2015 3,114 3 View
Hi! Can anyone suggest a systematic way to prepare activated carbon from raw materials? I've read journals which chemically impregnated their samples first before carbonisation through a furnace,...
15 October 2014 3,287 3 View
My sample needs to be heated in the microwave oven while being purged in gas, but the oven has no inlet for a gas source. Has anyone done this kind of modification? Thanks a lot!
29 May 2014 1,211 9 View