Bryan John Magoling

6 Questions 4 Answers 0 Followers

Questions related from Bryan John Magoling

Will intron-mediated enhancement of gene expression affect downstream of a fusion protein?

Hello everyone! I'm currently trying to increase the expression of my fusion protein in a C. elegans animal model. After reading papers, I found two possible methods to try: (1) codon-optimization...

30 September 2020 613 2 View

Why did stably expressing GFP outside the cell membrane resulted to low fluorescent signals?

Hi everyone. I have been trying to express my fusion protein to the outside membrane of the cell. My fusion protein contains eGFP. After lentivirus transduction, I observed a membrane-bound...

26 September 2020 4,924 2 View

Primers for amplifying Lactadherin?

Has anyone had previous success in amplifying Lactadherin from the addgene construct below? https://www.addgene.org/46830/ I'm looking at the sequence and it seems that the starting sequence of...

12 October 2019 1,278 3 View

What is the effect of moisture during microwave-assisted activation of lignocellulosic based carbon?

Does anyone know how moisture content can affect the activation process using microwave? For example, if I put char that is not completely dry and subject it to MW treatment, will the resulting...

12 April 2015 3,112 3 View

Which comes first in AC production: a carbonisation or chemical activation?

Hi! Can anyone suggest a systematic way to prepare activated carbon from raw materials? I've read journals which chemically impregnated their samples first before carbonisation through a furnace,...

15 October 2014 3,286 3 View

Does anyone know how to modify a regular microwave oven so that it can accomodate a continuous gas flow while heating my sample?

My sample needs to be heated in the microwave oven while being purged in gas, but the oven has no inlet for a gas source. Has anyone done this kind of modification? Thanks a lot!

29 May 2014 1,211 9 View