How to optimise the protocol for intracellular staining of ribosomal pS6 of human PBMC (mTORC1 downstream target)?

Hi, I am trying to stain human PBMC (population of interest is NK cells) for ribosomal pS6 and I go according to below mentioned steps but I do not get proper pS6 detection and also the surface...

25 June 2022 7,115 0 View