Brunette Frati

4 Questions 3 Answers 0 Followers

Questions related from Brunette Frati

How can I analyze the wound healing assay data if a 0 hour gap size of the created wound for treatment and control has different values?

Hi everyone , I did wound healing assay to measure the migration rate for HepG2 cells after transaction with miRNA and negative control . After cells reach 90% confluence, I made a wound (in...

06 January 2015 7,197 10 View

Can anyone help me with turbidity in cell culture media?

I am facing problem with cell culture, after I start the culture, the media turned turbid in about 2 days. I tried everything but the problem still persists. Under the microscope the cells look ok...

11 July 2014 2,953 9 View

Can I use Calcium Phosphate Transfection with miRNA ?

I am wondering if we can use Calcium Phosphate Transfection with miRNA. I want to transfect cancer cell line with miRNA to study the biological function of my target.

04 March 2014 9,160 0 View

Can anyone help me to do RT-PCR to analyze the expression of miRNA?

I am a new grad student and my project will be about miRNA. I am trying to do RT-PCR to analyze the expression of a specific miRNA in cell lines sample, but I am a little confused about how to run...

08 September 2013 6,082 2 View