Bruna Rodrigues

5 Questions 13 Answers 0 Followers

Questions related from Bruna Rodrigues

Do we have cell culture contamination?

We are experienced lately some problems with primary cells. We could see (even in 10x magnification) tiny bright particles around the addhered cells. There is no macroscopic sign of...

15 December 2016 7,543 11 View

Could anyone help me with PCR to Mycoplasma detection?

I'm tryint to standardize a PCR to mycoplasma detection of dna from cell culture. I'm testing samples positive for mycoplasma and negative as controls. I found a weak band unspecific slightly...

30 June 2016 7,899 3 View

Is there an effective protocol for cryopreservation of umbilical cord tissue?

Hi everyone, I'm looking for a good protocol for cryopreservation of umbilical cord tissue (wharton's jelly). Does anyone could help me with it? Do I have to cryopreserve the entire umbilical...

15 December 2015 5,956 2 View

Is there a protocol for Toluidine blue staining of chondrogenic differentiation of stem cells?

Hi everyone. Does exist a protocol to toluidine staining in monolayer cells? Or someone have a simple protocol to it?

11 December 2015 4,349 4 View

Could someone advise me about the best proportion of Ficoll-Paque Premium (1.077g) and bone marrow for isolation of mononuclear cells?

Hi, everyone. I want to isolate mononuclear cells from bone marrow using gradient centrifugation with Ficoll-Paque Premium 1.077g. However, GE's protocol suggests almost 1:1 of ficoll and bone...

22 October 2015 1,775 4 View