Why do I get no mRNA when substituting uridine for pseudouridine in an IVT reaction?

I have been following the IVT protocol as described in Henderson et al., “Cap 1 Messenger RNA Synthesis with Co-Transcriptional CleanCap® Analog by In Vitro Transcription.” This involves a 2.5hr...

03 November 2023 9,829 0 View