7 Questions 13 Answers 0 Followers
Questions related from Brittany Wingham
Hi, I am at CD8 cell priming following co-culture with stimulated murine DCs. I wondered if anyone could suggest a cell line for this, or might have any they would be willing to share? Many...
15 April 2016 4,345 1 View
Hi again! I've just started growing CTLL-2 IL2 dependent T cells in culture, and need quite high numbers. The ATCC recommendations for culture are to subculture actively growing cells BEFORE they...
12 February 2016 8,609 2 View
I have carried out some nanoparticle treatments on a set of cells, which I plan to use for western blot. Of 7 treatments, one set of cells has turned red, while another dark purple. I think this...
19 January 2016 5,636 7 View
As we know, the innate affinity between streptavidin and biotin is one of the strongest non-covelant interactions, with femtomolar affinity, and can resist changes in pH and temperature. My...
14 August 2015 5,949 3 View
Hi, I have been looking at formation of a peptide multimer bound to a streptavidin scaffold. The peptide is biotinylated, so should bind inherently with the streptavidin backbone. Currently, I...
29 June 2015 3,407 8 View
I have just begun western blots looking at CD40 in immune cell pull downs. The antibody daasheet states the band will be apparent at 41kDa, although the predicted size is 34kDa. I am not too...
25 June 2015 9,699 2 View
I have been using streptavidin as a constuct scaffold. Once solubilised in PBS, I have been having issues with fluctuations in the spectophotometer absorbance reading. An additional issue is that...
16 June 2015 9,836 2 View
