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Hi again! I've just started growing CTLL-2 IL2 dependent T cells in culture, and need quite high numbers. The ATCC recommendations for culture are to subculture actively growing cells BEFORE they...
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I have carried out some nanoparticle treatments on a set of cells, which I plan to use for western blot. Of 7 treatments, one set of cells has turned red, while another dark purple. I think this...
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Hi, I have been looking at formation of a peptide multimer bound to a streptavidin scaffold. The peptide is biotinylated, so should bind inherently with the streptavidin backbone. Currently, I...
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I have just begun western blots looking at CD40 in immune cell pull downs. The antibody daasheet states the band will be apparent at 41kDa, although the predicted size is 34kDa. I am not too...
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