Britt Merlaen

5 Questions 19 Answers 0 Followers

Questions related from Britt Merlaen

Correction for multiple testing in series of t-tests?

Dear all, I want to run a number of t-tests, each time comparing the effect of treatment x to the control on a particular timepoint in a particular tissue on gene expression. Since there are 4...

12 December 2016 7,426 8 View

Is this SDS-PAGE gel suited for Western Blot?

Dear all, I address my question in particular to people experience in western blot on membrane proteins.. I treat my plasma membrane extracts by adding a (laemmli) sample buffer heavy in...

02 February 2016 9,749 7 View

Can anyone provide me with a biochemical step-by-step protocol to determine (vanadate-sensitive) ATPase in a membrane extract?

I would like to determine the purity of my plasma membrane extract from plant material. Can anyone tell me how to determine e.g. vanadate-sensitive ATPase or other markers? Or would you recommend...

10 October 2015 1,355 2 View

How to interprete this gel?

Hi! Are these bands primer dimers or not? lanes 1 -> 7: sampleslane 8: negative controllane 9: generuler 100bp plus (lowest band of ladder = 100bp)lane 10-> 16: sample = 1 µl from PCR...

08 August 2014 2,728 9 View

Any suggestions on primers design for 5' RACE PCR?

Hi, I am designing gene specific primers for 5' RACE PCR. I am using the protocol in the attachment. I can see that the GSP-RT primer needs to be antisense regarding the mRNA sequence, but do GSP1...

07 July 2014 2,634 4 View