Britt Merlaen

4 Questions 16 Answers 0 Followers

Questions related from Britt Merlaen

Is this SDS-PAGE gel suited for Western Blot?

Dear all, I address my question in particular to people experience in western blot on membrane proteins.. I treat my plasma membrane extracts by adding a (laemmli) sample buffer heavy in...

08 February 2016 9,812 6 View

Can I store ascorbic acid stock in the freezer?

Hi, I want to use ascorbic acid as a protease inhibitor. Can I make a stock (in water) and store aliquots at -20°C? Or is it still prone to degradation due to presence of oxygen then?

07 October 2015 7,210 9 View

Which program can I use to manually color or highlight specific residues in an alignment?

I have a protein alignment and I would like to highlight specific regions and specific residues. Can I do this with CLC Sequence Viewer? If not, which program can do this?

10 July 2015 3,845 21 View

Any suggestions on primers design for 5' RACE PCR?

Hi, I am designing gene specific primers for 5' RACE PCR. I am using the protocol in the attachment. I can see that the GSP-RT primer needs to be antisense regarding the mRNA sequence, but do GSP1...

31 July 2014 595 3 View