Brindha Chelliappan

2 Questions 5 Answers 0 Followers

Questions related from Brindha Chelliappan

How to transport rprotein from E.coli? frozen,glycerol,lyopilized which is stable without protein denaturation?

how to transport rprotein from E.coli? frozen,glycerol,lyopilized which is stable without protein denaturation?

23 January 2017 3,371 3 View

How to increase the binding capacity of His tagged recombinant protein to Ni NTA resin?

I am trying to purify my 46kDa His tagged recombinant protein from BL21 E.coli cells lysate with Ni NTA resin using 10mM and 20mM Imidazole concentration in the wash buffer. But all the protein...

01 April 2015 5,664 12 View