Brijesh Dabhi

4 Questions 13 Answers 0 Followers

Questions related from Brijesh Dabhi

Can anyone help me calculate the concentration of calcium from serum sample?

I have got O.D. for estimating Ca from serum by spectrophotometer. Does anyone have molar extinction coefficient of Ca estimation or can give me values of standard conc. of Ca.?

13 November 2013 1,973 2 View

Has anyone used RT PCR of Corbett company?

We have Corbett's RT PCR in our lab but I am having difficulty in analysis of data and I am getting amplification in negative control. Can anyone help me ?

09 March 2013 9,785 6 View

How to calculate the Ct value after an RT-PCR run?

I want to study expression analysis of cytokines, for which I have run an RT-PCR. I am using a corbett rotor gene 6000 system. But the thing is that I don't know how to analyze the data obtained...

18 February 2013 6,584 3 View

How can I determine the genotype of samples?

I want to determine SNP of control and diabetic patients. I have designed primers for three genes and amplified their DNA with PCR. How can I determine the genotype of these samples after that?

29 November 2012 1,868 24 View