Does the RNA/cDNA needs to be mixed for qPCR or can the whole plate be vortexed at slow speed to achieve the same?

This question is for Taqman qPCR assay containing FAM/TAMRA probe for amplification of Viral RNA. While pipetting RNA/cDNA in 96-well plate, I always mix it couple of times to ensure mixing of...

01 March 2022 1,426 3 View