Brij Nath Tewari

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Questions related from Brij Nath Tewari

Could anyone provide some reasons why after a ligation-transformation exp.I get equal no. of colonies on control and Exp. ampicillin resistant plates?

I am doing a ligation with a 3.8kb vector and inserts are 672 bp. The vector is ampicillin resistant. I got every time sharp band (sharp band linearazed plasmid DNA and insert band) on gel from...

01 January 2018 1,651 5 View

How to isolate RNA from breast cancer tissue.

I am looking for a proper protocol for RNA extraction from breast cancer tissue. I conducted a number of trials and was unsuccessful. I got RNA quantity, concentration = 100ng/ul 260/280=...

01 January 2013 8,970 4 View