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Questions related from Brianne Jeffrey
I am trying to section rat brains for IHC. Typically, we perfuse transcardially with 4% PFA, post-fix in 4% PFA, and store in 30% sucrose before sectioning on a cryostat. I am trying to label a...
20 January 2015 8,475 6 View
One antibody stains soma and the other stains a diffuse protein, located throughout the cells in soma and neurites. We don't see fluorescent overlap, but how can I quantify this? The protein...
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