Brian Vogler

3 Questions 10 Answers 0 Followers

Questions related from Brian Vogler

Is the smear caused by concentrated DNA indicative of any real problems?

I am digesting and concentrating DNA for transformation, but with my more concentrated samples, the agarose gel smears with what appear almost like bubbles at heavier weights. I want to know if...

04 April 2014 3,092 11 View

Appearance of new, large band after treatment of purified RNA with RNase?

I treated a purified RNA sample with DNase and also with RNase (separate). The DNase-treated prep looked identical to untreated (as expected), but the RNase-treated prep disappeared (expected)...

10 October 2013 1,935 14 View

What are the essential benchmark quantities for inverse PCR?

In the protocols I've found, important quantities are either omitted (volumes of DNA w/o concentration) or useless to me (0.5 flies of DNA). I'd like to know values people have found to work for:...

08 August 2013 7,938 5 View