Brian Mcgillick

4 Questions 16 Answers 0 Followers

Questions related from Brian Mcgillick

Does anyone have a protocol for binding streptavidin to a 96 well plate?

I realize you can buy streptavidin coated plates, but money is a little tight in the lab right now so I was hoping to find a more cost efficient method since we go through these plates very fast.

04 April 2015 9,033 6 View

Could someone explain how fig. 1a of the attached paper demonstrates a Kcat determination?

They seem to simply be plotting the rate of formation of product at multiple substrate concentrations, however they are well below the enzyme Km (they only go to 60uM when the Km is approx. 1mM)....

03 March 2015 5,231 3 View

What are some possible causes of an unusually high Km Value?

I am performing a very straightforward continuous assay where I basically follow a decrease in absorbance which corresponds to the conversion of my substrate to its product. However, I am getting...

10 October 2014 6,083 12 View

Why is my protein getting lost in the Nickel Column after On-Column Refolding attempts?

I have a protein that I'm attempting to refold on a Nickel Column. I have already confirmed that the protein is soluble in urea and that the protein binds strongly to the Nickel column (I just...

06 June 2014 3,551 4 View