Brian A. Dow

4 Questions 6 Answers 0 Followers

Questions related from Brian A. Dow

XL-10 Gold E. coli transofrmation procedure with Beta-mercaptoethanol not provided by Agilent?

I am using XL-10 gold cells for transformation following PCR. Normally I use the B-mercaptoethanol provided with the cells by Agilent. However, I have run out of it, and I was wondering if...

12 December 2014 6,504 4 View

How to use Ellman's reagent (DTNB) for determination of Tm of disulfide?

I have a beta barrel protein with a single interior disulfide. I would like to use Ellman's reagent (DTNB) to determine at what temperature the disulfide breaks via the absorbance of TNB at 412...

09 September 2014 2,192 5 View

How can roGFP be targeted to the periplasm?

I am designing an experiment to measure the effect of a mutant electron transfer (ET) mediating protein on the production of ROS. The ET mediator is located in the periplasm, and I am expressing...

02 February 2014 1,968 1 View

How can I purify a protein without losing its chemical ligand?

I have a blue copper protein, which I suspect may be binding an external chemical ligand in vivo. I am concerned that I may be losing this chemical ligand during purification. I normally purify...

02 February 2014 3,557 2 View