Brian G. O' Flynn

4 Questions 2 Answers 0 Followers

Questions related from Brian G. O' Flynn

What is the relationship between Km and Kd for a bisubstrate reaction?

It is known that Km = (k2 + k-1)/(k1) and Kd = (k-1)/(k1) for a simple unimolecular conversion of E + S ---> ES -----> E + P, but what about the relationship for an ordered sequential bi bi...

15 March 2018 5,455 0 View

Can anyone help me with an enzyme kinetics sigmaplot fit?

I've plotted my data and it looks to be good for a random sequential mechanism. When plotted in excel it fits this trend nicely. However, when I plot these in SigmaPlot, it does not want to put...

09 June 2017 5,985 3 View

Protein purification/concentration using ZipTips?

Has anyone ever used ZipTips to concentrate or purify a protein? I can't think of any reason why this wouldn't work if you use the correct tip and solvent. As a benchmark, my protein is 23 kDA....

30 March 2017 2,538 1 View

When forming a phylogenic tree (cladogram) using MEGA, what kind of model would best suit?

So I work in an enzymology lab in a chemistry dept. We do not have much experience with cladistic analysis, however it was suggested by a reviewer of a paper that it would help some readers,...

30 January 2017 7,321 4 View