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Questions related from Brian G. O' Flynn
It is known that Km = (k2 + k-1)/(k1) and Kd = (k-1)/(k1) for a simple unimolecular conversion of E + S ---> ES -----> E + P, but what about the relationship for an ordered sequential bi bi...
15 March 2018 5,487 0 View
I've plotted my data and it looks to be good for a random sequential mechanism. When plotted in excel it fits this trend nicely. However, when I plot these in SigmaPlot, it does not want to put...
09 June 2017 6,019 3 View
Has anyone ever used ZipTips to concentrate or purify a protein? I can't think of any reason why this wouldn't work if you use the correct tip and solvent. As a benchmark, my protein is 23 kDA....
30 March 2017 2,562 1 View
So I work in an enzymology lab in a chemistry dept. We do not have much experience with cladistic analysis, however it was suggested by a reviewer of a paper that it would help some readers,...
30 January 2017 7,388 4 View
I recently ligated a gene about 700 bp long into pET-28a using Xho-I and Nde-I restriction enzymes. The first time I attempted transformation into XL-10 cells on kanamycin plates I got a handful...
07 September 2016 7,995 9 View
