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Questions related from Brian Mcgillick
I realize you can buy streptavidin coated plates, but money is a little tight in the lab right now so I was hoping to find a more cost efficient method since we go through these plates very fast.
02 April 2015 4,274 9 View
I am developing a plate based assay and due to the limitations of the plate binding sites there is an upper limit to how much substrate I can assay. I would like to calculate Km but the maximum...
17 February 2015 9,810 11 View
More specifically, I have a simple enzymatic assay where I follow the Absorbance at 260 nm as my substrate is converted to product. Based on the literature values of extinction coefficients, there...
22 January 2015 6,296 11 View