Brian Mcgillick

3 Questions 18 Answers 0 Followers

Questions related from Brian Mcgillick

Does anyone have a protocol for binding streptavidin to a 96 well plate?

I realize you can buy streptavidin coated plates, but money is a little tight in the lab right now so I was hoping to find a more cost efficient method since we go through these plates very fast.

02 April 2015 4,270 9 View

Can Km be faithfully calculated if all tested substrate concentrations are below Km?

I am developing a plate based assay and due to the limitations of the plate binding sites there is an upper limit to how much substrate I can assay. I would like to calculate Km but the maximum...

17 February 2015 9,807 11 View

What is a possible cause for a larger than expected change in absorbance? Or restated, a much lower extinction coefficient than expected?

More specifically, I have a simple enzymatic assay where I follow the Absorbance at 260 nm as my substrate is converted to product. Based on the literature values of extinction coefficients, there...

22 January 2015 6,293 11 View