Brian Hardaway

3 Questions 3 Answers 0 Followers

Questions related from Brian Hardaway

Are TUNEL-postive, DAPI-negative cells real or false-positives?

I am doing in situ TUNEL staining on mouse tissue sections. I see a fair number of bright TUNEL-positive, DAPI-negative events in many sections. I have seen in some papers that cells lose DAPI in...

21 September 2021 4,103 3 View

How to Balance Bench work with Reading and Writing?

I am a second-year grad student. I am finding it difficult finding time to read literature and write (things like qualifying exam, thesis, grants). I find myself so busy with bench work that I...

02 March 2021 3,604 4 View

Purified Protein at 4 degrees celsius for roughly 36 hours?

I purified a mutant version of EF-Tu (E348C) that has a his-tag and an amino acid sequence that TEV protease cleaves to remove the his-tag. I purified by Ni-NTA column and added about 40%...

15 December 2018 5,580 3 View