Brett Arenberg

3 Questions 1 Answers 0 Followers

Questions related from Brett Arenberg

What's the best way to CRISPR edit primary human CD4+ T cells?

We are isolating CD4+ human primary T-cells from PBMC using immunomagnetic isolation (negative selection). We are transfecting them with our sgRNA using electroporation, but we are getting really...

12 June 2025 819 0 View

Is secondary stimulation in human T-helper differentiation necessary?

I noticed in a few papers that after they culture the T-cells with TCR activators and differentiating cytokines for 4-6 days, they wash away the cytokines and stimulate for another day with just...

01 April 2025 9,509 1 View

How do I siRNA to knockout a gene in frozen human T-Cells?

We are trying to knockout a gene in previously frozen human T cells using siRNA. The cells are isolated from human blood and separated from PBMC using negative selection immunomagnetic isolation....

06 December 2024 6,396 1 View