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Questions related from Brett Arenberg
We are isolating CD4+ human primary T-cells from PBMC using immunomagnetic isolation (negative selection). We are transfecting them with our sgRNA using electroporation, but we are getting really...
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I noticed in a few papers that after they culture the T-cells with TCR activators and differentiating cytokines for 4-6 days, they wash away the cytokines and stimulate for another day with just...
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We are trying to knockout a gene in previously frozen human T cells using siRNA. The cells are isolated from human blood and separated from PBMC using negative selection immunomagnetic isolation....
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