Brendan Delange

2 Questions 2 Answers 0 Followers

Questions related from Brendan Delange

How can I increase the specificity of long-tail PCR primers?

I'm using tailed primers: 20-nt of priming sequence followed by a 10-nt tail. I know to use the annealing temperature (T_A) of the priming sequence (not the full 30-nt sequence) when designing my...

21 December 2014 6,208 7 View

How long can un-purified PCR reactions be stored at -20C after thermocycling?

The polymerase system I am using is Thermo Phusion Hot-Start II, a pfu-based recombinant Pol with a reversible affibody. The reason that I don't want to purify the reaction mixtures after...

05 December 2014 1,087 3 View