Bratati Mukherjee

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Questions related from Bratati Mukherjee

Overexpressing a protein of interest as an ubiquitinylated fusion protein but how to prevent protein and ubiquitinase from precipitating out?

The protein is a small soluble protein around 14 Kda and is precipitating copiously beyond the deubiquitinylation step, especially on spin concentration. It is used in the pHUE vector system. When...

06 June 2016 4,161 1 View

Increasing yield of protein in BL21 DE3

I am trying to overexpress a 13 kDa soluble protein in BL21-DE3. So far I am getting very poor yields (0.7 mg/Liter) and am needing tonnes of cultures to have enough protein for ITC and...

06 June 2016 3,004 3 View