Bram Piersma

2 Questions 4 Answers 0 Followers

Questions related from Bram Piersma

Can you stain live/dead cells in 3D tissue culture?

Hi Everyone, I am optimising a ex-vivo 3D culture of human tissues of +/- 300 um thickness. We want to know how many cells are alive and how many are dead at specific timepoints. I know I could...

12 December 2019 9,338 3 View

Why do my primary fibroblasts look enlarged with granules and debris in the medium after Lentiviral transduction?

Hi all, I'm trying to overexpress a protein of interest in a pCDH-CMV-GOI-EF1-copGFP vector in primary human fibroblasts. The problem I have is that a couple of days after transduction, my cells...

03 March 2015 172 6 View