Bram Piersma

3 Questions 8 Answers 0 Followers

Questions related from Bram Piersma

Can you stain live/dead cells in 3D tissue culture?

Hi Everyone, I am optimising a ex-vivo 3D culture of human tissues of +/- 300 um thickness. We want to know how many cells are alive and how many are dead at specific timepoints. I know I could...

04 December 2019 686 3 View

Can you recommend transcript variant specific siRNA?

Hi Everybody, I am aiming to specifically knock down transcript variants of my GOI. Can anyone recommend vendors that design transcript variant specific siRNA? Please only reply if you have...

11 October 2019 9,547 0 View

How to isolate chromatin for ChIP from 3D collagen gels?

Hi everybody, I am planning to perform ChIP experiments and sequencing on fibroblasts that reside in a 3D collagen/matrigel gel. Does anyone have experience with fixing and subsequent harvesting...

30 January 2019 2,523 3 View