Bram Geenen

2 Questions 3 Answers 0 Followers

Questions related from Bram Geenen

How do I reduce background when doing IHC on mouse tissue using a mouse primary antibody?

We have a mouse model for Alzheimer's disease which is expressing human amyloid beta in plaques. We want to detect those using a mouse anti-amyloid beta antibody, using a fluorescently labeled...

10 November 2022 7,219 3 View

Can anyone comment on my protocol for making an ISH probe without using bacteria?

In my lab we're doing in situ hybridization on brain sections of mice & rats. However, we don't have the facilities to generate DIG-labeled probes using the usual procedure (generating a plasmid...

13 May 2014 5,584 3 View