Whole cell IPSC/EPSC recordings from CA3- What internal solution should I use: Cs v K-gluconate, Cl-concentration?

Hi, I have been doing EPSC/IPSC recording from CA3 pyramidal neurons in acute slices using a CsGluconate internal solution (in mM):  130 Cs-Gluconate, 10 HEPES, 0.6 EGTA, 3 MgCl2, 1 NaCl, 0.4...

14 October 2015 9,787 7 View