Brad Huffington

3 Questions 4 Answers 0 Followers

Questions related from Brad Huffington

Western blotting (with ECL reagent and CCD imager): What is the optimum exposure time for bands for quantification?

I'm quantifying proteins on western blots, using chemiluminescent ECL primer and a CCD-based imager (Carestream) to visualize the bands. I can use exposure times of seconds to minutes. The shorter...

18 April 2014 5,561 2 View

How long can you store SDS-PAGE gels before use?

Sometimes I prepare a standard SDS-PAGE gel for westerns, wrap with a wet paper towel and cling wrap, and store it in a zip lock baggy in the fridge. Does storage for a few days alter the...

07 March 2013 9,805 31 View

How to get rid of high background + non-specific negative ("ghost") bands on a western blot of a tissue homogenate?

The antibody (and exact protocol) that I'm using worked in the recent past, but now I get very high background, along with a "ladder" of negative ghost bands against the dark background. My...

24 January 2013 5,896 11 View