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My dear all, I purified trx tagged protein, but to cleave the tag I has to use enterokinase which works in the Tris buffer. My protein was in phosphate buffer.how can I transfer my desired protein...
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Dear all, I'm working with protein purification, the protein has a theoretical pI of 5.95 along with TRX tag. can any one suggest me which buffer has to use for maximum protein elution with...
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