Boris Klimovich

4 Questions 8 Answers 0 Followers

Questions related from Boris Klimovich

Which medium is suitable for short-term culture and lentiviral infection of murine AML cells?

I need an advice on medium which will support AML blasts from mouse for around 1 week. I also want to infect cells with lentivirus and, if possible, select them with puromycin. Does anyone have an...

20 March 2016 5,323 1 View

Are Plat E cells better than Phoenix for retrovirus packaging?

I am packaging MSCV virus with Phoenix Eco, but titers are low - about 10^5, inspite of good transfection efficiency. As a result I have quite low and inconsistent results of fetal liver cells...

16 July 2015 6,525 3 View

In a Co-infection with 2 retroviruses should the packaging be performed separately or not?

Dear colleagues, I am performing an infection of HSC with retroviruses (MSCV-based vector). According to paper I am reproducing, viruses are packaged separately and supernatants are mixed right...

06 March 2015 5,400 10 View

What is the cause of low transfection efficiency with bacmid?

Dear colleagues,We need some suggestions about baculovirus system. We utilize Bac-toBac HBM secreted system (Invitrogen to express our protein). For transfection Cellfectin 2 is used. The problem...

02 October 2014 2,731 10 View