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I need an advice on medium which will support AML blasts from mouse for around 1 week. I also want to infect cells with lentivirus and, if possible, select them with puromycin. Does anyone have an...
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I am packaging MSCV virus with Phoenix Eco, but titers are low - about 10^5, inspite of good transfection efficiency. As a result I have quite low and inconsistent results of fetal liver cells...
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