Boris Bokor

2 Questions 6 Answers 0 Followers

Questions related from Boris Bokor

Measuring concentration of cDNA:RNA hybrid as ssDNA od dsDNA?

Hi everyone, After reverse transcription, I need to measure cDNA:RNA hybrid quantification befor qPCR analysis. I do not know, if I should measure ssDNA or dsDNA? Many people here had similar...

11 September 2017 5,470 2 View

Bimodal melting curve in qPCR

Hi all, what is the solution for bimodal melting curve in qPCR? This double peak is slight in let´s say A samples, but terrible in B samples. What could be the reason for that? GC content of the...

07 July 2017 5,016 5 View