Boon Lee Chai

6 Questions 15 Answers 0 Followers

Questions related from Boon Lee Chai

Can anyone help in FFPE PCR for a difficult gene region?

I am trying to amplify a gene region from FFPE. I have try normal conventional method mixing everything together to master mix. Attached is the image of using conventional method and 3 different...

22 May 2015 6,104 10 View

Is FFPE DNA unstable for conventional PCR?

I am trying to amplify target sequence using FFPE DNA. Initially, I managed to get the target band I want although it is weak. However, when I try to repeat the test using same formula, no band...

02 January 2015 4,792 8 View

Does anyone have good primers for APC gene at MCR?

I am using FFPE samples to detect APC mutation at MCR. Are there any good papers I can refer my work to?

26 September 2014 529 0 View

How to score the epithelial section of intestine for immunohistochemistry?

I am confused with scoring the epithelial sections of intestine because there is the vertical and longitudinal section. Which should I score if both structure exist?

18 September 2014 7,131 7 View

Can anyone suggest a good anti-APC C-terminal antibody?

Besides HT29 as the negative control for anti-APC C-terminal antibody, is there any other colorectal cell lines that can be used? Could we possibly use SW480?

04 June 2014 9,925 2 View

How to detect chromosomal Instability(CIN)? What are the methods available?

I am doing cancer study and I might need to do CIN. Is there any reference, journals, books available that can give me some ideas?

13 January 2014 8,295 6 View