What is the possible reasons for getting this kind of melt curve in NTC (no template control)?

I ran GAPDH NTC using SYBR Green and I got this fuzzy melt curve which I am not sure what happened. My master mix (MM) reaction for NTC is 15 microlit MM+ 5 microlit H20. Will the amount of water...

12 July 2016 7,600 3 View